Fate of a Major Soybean Allergen, Gly m Bd 30K, in Rice-, Barley- and Soybean-Koji Miso (Fermented Soybean Paste) during Fermentation.
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چکیده
منابع مشابه
Identification of the glycosylation site of a major soybean allergen, Gly m Bd 30K.
A major soybean allergen, Gly m Bd 30K, is a glycoprotein. A peptide containing a sugar chain was isolated from an alpha-chymotrypic hydrolyzate of the allergen. The amino acid sequence analysis of the peptide showed that its sugar chain binds to the Asn170 residue. Furthermore, the sugar moiety of the allergen and peptide was shown to consist of mannose, N-acetylglucosamine, fucose, and xylose...
متن کاملIdentification of the soybean allergenic protein, Gly m Bd 30K, with the soybean seed 34-kDa oil-body-associated protein.
The soybean allergenic protein, Gly m Bd 30K [Ogawa et al., J. Nutr. Sci. Vitaminol., 37, 555-565 (1991)] which is most strongly and frequently recognized by the IgE antibodies in sera of soybean-sensitive patients with atopic dermatitis, has been characterized. The allergen was isolated from the crude 7S-globulin fraction as an oligomeric form with a molecular weight of more than 3000,000 by g...
متن کاملNoteMeasurement of Gly m Bd 30 K , a Major Sandwich Enzyme - linked ImmunosorbentSoybeanAssayAllergen , in Soybean Products
derrnatitis, we found 16 allergenic proteins in the soybean. i) About two-thirds of soybean-sensitive patients examined was shown to have IgE antibodies binding to the 30-kDa protein narned Gly m Bd 30K. This finding suggests that Gly m Bd 30K is a major allergen in the soybean and that it may be a target allergen for hypoallergenization of soybean products. Gly m Bd 30K has been purified to ho...
متن کاملSoybean allergy: IgE epitopes of glycinin (Gly m 6), an important soybean allergen
Methods 11 subjects with clinically confirmed soybean allergy and sIgE against rGly m 6 were enrolled in this study. Synthetic overlapping peptides (15-mers, 4 aa offset) representing the amino acid sequences of G1 and G5, two representative subunits of Gly m 6, were spotted onto CelluSpotTM slides and analyzed for IgE-binding, respectively. The sequences of IgE-binding peptides were allocated ...
متن کاملFunctional Characterization of Bacterial Communities Responsible for Fermentation of Doenjang: A Traditional Korean Fermented Soybean Paste
Doenjang samples were prepared in triplicate and their microbial abundance, bacterial communities, and metabolites throughout fermentation were analyzed to investigate the functional properties of microorganisms in doenjang. Viable bacterial cells were approximately three orders of magnitude higher than fungal cells, suggesting that bacteria are more responsible for doenjang fermentation. Pyros...
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ژورنال
عنوان ژورنال: Food Science and Technology International, Tokyo
سال: 1997
ISSN: 1881-3976,1341-7592
DOI: 10.3136/fsti9596t9798.3.145